Pseudoviruses are useful surrogates for highly pathogenic viruses because of their safety, genetic stability, and Cake Stands scalability for screening assays.Many different pseudovirus platforms exist, each with different advantages and limitations.Here we report our efforts to optimize and characterize an HIV-based lentiviral pseudovirus assay fo
Mechanical ventilation in the ICU- is there a gap between the time available and time used for nurse-led weaning?
Abstract Background Mechanical ventilation (MV) is a key component in the care of critically ill and injured patients.Weaning from MV constitutes a major challenge in intensive care units (ICUs).Any delay in weaning may increase the number of First Aid Kits complications and leads to greater expense.Nurse-led, protocol-directed weaning has become p
Behavioural adaptations to moisture as an environmental constraint in a nocturnal burrow-inhabiting Kalahari detritivore Parastizopus armaticeps Peringuey (Coleoptera: Tenebrionidae)
The nocturnal desert detritivore Parastiz.opus armaticeps shows differences in surface activity patterns and burrow fidelity depending on surface Hand Grip Set humidity.After rain approximately half of the beetle population, independent of sex, is highly vagile and disperses over long distances.During drought, beetles are more sedentary and show hi
Fast and deep phosphoproteome analysis with the Orbitrap Astral mass spectrometer
Abstract Owing to its roles in cellular signal transduction, protein phosphorylation plays critical roles in myriad cell processes.That said, detecting and quantifying protein phosphorylation has remained a challenge.We describe the use of a novel mass spectrometer (Orbitrap Bike Accessories - Computers Astral) coupled with data-independent acquisi